Experimental techniques to investigate the structures of fuzzy complexes

X-ray:X-ray crystallography
NMR:Nuclear magnetic resonanceFRET:Förster resonance energy transfer
PRE:paramagnetic relaxation enhancementsmFRET:single molecule FRET
EM:electron microscopyH/D exchange:hydrogen deuterium exchange
CD:circular dichroismMS:mass spectroscopy
FS:fluorescence spectroscopyEPR:electron paramagnetic resonance
AUC:analytical ultracentrifugationSEC:size-exclusion chromatography
SAXS:small-angle X-ray scatteringSPR:surface plasmon resonance
FTIR:Fourier transform infrared spectroscopyITC:isothermal titration calorimetry
UV-VIS:ultraviolet-visible spectroscopyLS:light scattering
AFM: atomic force microscopyH/D:H/D exchange
HS-AFM:high speed atomic force microscopyFPA:fluorescence polarization analysis
Y2H:yeast two hybrid screeningFA:fluorescence anisotropy

Functional analysis techniques

TA:transcriptional assay
SDM:site-directed mutagenesis
PROT:proteolysis
CYT:cytoduction